Turnover Rate of Hepatic 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase as Determined by Use of Cycloheximide
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چکیده
منابع مشابه
Purification of 3-hydroxy-3-methylglutaryl coenzyme A reductase.
The enzyme 3-hydroxy-3-methylglutaryl coenzyme agarose, glucose-6-phosphate dehydrogenase, and aldolA reductase from human liver was purified to homogeneity. ase were from Sigma Chemical co.; agarose-hexaneHMG-CoA was from P-L Biochemicals; bovine serum Electrophoresis of the purified human enzyme on non-denaturing gels revealed a single protein-staining band that co-migrated with reductase act...
متن کاملRegulation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol synthesis.
Rat liver microsomal 3-hydroxy-3-methylglutaric acid (HMG)-coenzyme A reductase (mevalonate:NADP oxidoreductase (acylating CoA), EC 1.1.1.34) exhibits a cyclic rhythm with peak activity at midnight. HMG-CoA reductase from microsomes of rats killed at noon and midnight has similar properties. Cycloheximide completely prevents the 5to IO-fold rise in activity which occurs from 6 p.m. to midnight,...
متن کاملImproved methods for the solubilization and assay of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase.
A method for solubilizing HMG-CoA reductase is described that reproducibly yielded approximately 190% of the activity assayed in rat liver microsomes. Optimal solubilization occurred when microsomal membranes were frozen at a fixed concentration, thawed, homogenized in a buffer containing 50% glycerol, and incubated at 37 degrees C for 60 minutes. A rapid spectrophotometric assay of the reducta...
متن کاملHepatic cholesterol metabolism in obesity: activity of microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Obesity is often associated with an elevated total body cholesterol synthesis. In order to evaluate the role of hepatic cholesterogenesis in this phenomenon, we assayed the rate-limiting step in cholesterol biosynthesis, 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase in the microsomal fraction of liver biopsies obtained operatively from ten morbidly obese (relative body weight greate...
متن کاملLiver-specific deletion of 3-hydroxy-3-methylglutaryl coenzyme A reductase causes hepatic steatosis and death.
OBJECTIVE 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) catalyzes the rate-limiting step in cholesterol biosynthesis and has proven to be an effective target of lipid-lowering drugs, statins. The aim of this study was to understand the role of hepatic HMGCR in vivo. METHODS AND RESULTS To disrupt the HMGCR gene in liver, we generated mice homozygous for a floxed HMGCR allele and het...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)45588-9